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To investigate how the presence of seminal plasma affects human sperm capacitation, we used a proteomics approach to analyze changes that occur in the sperm proteome during capacitation. Our approach consisted of 3 steps: (i) the identification of proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and two-dimensional electrophoresis of purified spermatozoa incubated in either seminal plasma or artificial media; (ii) the determination of the capability of some of the identified proteins to capacitate spermatozoa; and (iii) the comparison of the identified proteins in seminal plasma with those identified in bovine seminal plasma and artificial media. By these procedures, we have identified 37 proteins that are present in human but not in bovine seminal plasma or artificial media, and 14 proteins that are present in human but not in bovine seminal plasma. Also, we have identified proteins that are present in human and bovine seminal plasma, but absent in artificial media. Our results suggest that capacitation is a complex process, involving the participation of more than one protein. Furthermore, the proteins identified in human spermatozoa that were identified in human seminal plasma may provide molecular markers to distinguish between acrosome-reacted and capacitated spermatozoa.A trial of venlafaxine and imipramine in the treatment of depression in patients with chronic pain. There is considerable evidence that depression is common in patients with chronic pain. Although both the development of depression and the response to antidepressants may be related to the presence of chronic pain, the influence of chronic pain on the response to antidepressants has not been investigated. We compared venlafaxine and imipramine in the treatment of depressive symptoms in patients with chronic pain and depression. In a parallel group design, 49 patients with chronic pain and major depression were randomized to receive one of two 8-week treatments with imipramine or venlafaxine. Depression was assessed with the Hamilton Depression Rating Scale (HDRS), and the pain intensity was measured with an 11-point numerical rating scale. Venlafaxine and imipramine were equally effective in reducing depression (HDRS scores) and pain (numerical rating scores). The side effect profile and tolerability of both drugs were similar. The results suggest that the response to antidepressant treatment is not affected by the 1a423ce670 NooBoss For Chrome 4.4.0 Torrent (Activation Code) (2022) What's New in the? 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